Rheumatology Division Proteomics Group - ProteoRed ISCIII

Instituto de Investigaciones Biomédicas de A Coruña-Complexo Hospitalario Universitario de A Coruña (INIBIC-CHUAC)
As Xubias, 84; 15006 A Coruña
Phone: +34 981176399; Fax. +34 981176398


Team

Francisco Javier Blanco García (Principal Investigator)

Jesús Mateos Martín, PhD (Postdoctoral Researcher)
Nieves Domenech García  (Biobank Facility Coordinator)
Arancha Landeira Abia  (PhD Student)


Research Lines

Laminopathies

Aeging
Stem cells
Proteomics


Equipment and facilities

Proteomics Facility:

Two Tempo nanoLC system (Eksigent)
1200 HPLC system (Agilent)
4800 MALDI-TOF/TOF system (ABSciex) for Peptide Fingerprinting, SILAC and iTRAQ protein relative quantitation
5500 Q-TRAp system (ABSciex) for Multiple Reaction Monitoring (MRM) absolute protein quantitation


Genomics facility

3130x1 Genetic Analyzer (Applied Biosystems)
Real-Time LigthCycler 480 (Roche)


Cell sorting facility

BD FACSAria (flow cytometry)
BD FACSCalibur (Cell sorting)


Biobank facility

Robotic dispensers (Freedom Evo)
Robotic Freezers (-20 ºC and -80 ºC)


Publications
  • De la Fuente A, Mateos J, Lesende-Rodríguez I, Calamia V, Fuentes-Boquete I, de Toro FJ, Arufe MC, Blanco FJ. Proteome analysis during chondrocyte differentiation in a new chondrogenesis model using human umbilical cord stroma mesenchymal stem cells. Mol Cell Proteomics. 2012 Feb;11(2):M111.010496. doi: 10.1074/mcp.M111.010496. Epub 2011 Oct 17. PubMed PMID: 22008206; PubMed Central PMCID: PMC3277753.
  • Fernández-Puente P, Mateos J, Fernández-Costa C, Oreiro N, Fernández-López C, Ruiz-Romero C, Blanco FJ. Identification of a panel of novel serum osteoarthritis biomarkers. J Proteome Res. 2011 Nov 4;10(11):5095-101. doi: 10.1021/pr200695p. Epub 2011 Oct 19. PubMed PMID: 21973172.
  • Cillero-Pastor B, Mateos J, Fernández-López C, Oreiro N, Ruiz-Romero C, Blanco FJ. Dimethylarginine dimethylaminohydrolase 2, a newly identified mitochondrial protein modulating nitric oxide synthesis in normal human chondrocytes. Arthritis Rheum. 2012 Jan;64(1):204-12. doi: 10.1002/art.30652. PubMed PMID: 21898353.
  • Mateos J, Lourido L, Fernández-Puente P, Calamia V, Fernández-López C, Oreiro N, Ruiz-Romero C, Blanco FJ. Differential protein profiling of synovial fluid from rheumatoid arthritis and osteoarthritis patients using LC-MALDI TOF/TOF. J Proteomics. 2012 Jun 6;75(10):2869-78. doi: 10.1016/j.jprot.2011.12.042. Epub 2012 Jan 8. PubMed PMID: 22245418.
  • Calamia V, Rocha B, Mateos J, Fernández-Puente P, Ruiz-Romero C, Blanco FJ. Metabolic labeling of chondrocytes for the quantitative analysis of the interleukin-1-beta-mediated modulation of their intracellular and extracellular proteomes. J Proteome Res. 2011 Aug 5;10(8):3701-11. doi: 10.1021/pr200331k. Epub 2011 Jul 5. PubMed PMID: 21692455.
  • Arufe MC, De la Fuente A, Mateos J, Fuentes I, De Toro FJ, Blanco FJ. Analysis of the chondrogenic potential and secretome of mesenchymal stem cells derived from human umbilical cord stroma. Stem Cells Dev. 2011 Jul;20(7):1199-212. doi: 10.1089/scd.2010.0315. Epub 2010 Dec 29. PubMed PMID: 20977334.
  • Calamia V, Ruiz-Romero C, Rocha B, Fernández-Puente P, Mateos J, Montell E, Vergés J, Blanco FJ. Pharmacoproteomic study of the effects of chondroitin and glucosamine sulfate on human articular chondrocytes. Arthritis Res Ther. 2010;12(4):R138. doi: 10.1186/ar3077. Epub 2010 Jul 13. PubMed PMID: 20626852; PubMed Central PMCID: PMC2945029.
  • Ruiz-Romero C, Calamia V, Rocha B, Mateos J, Fernández-Puente P, Blanco FJ. Hypoxia conditions differentially modulate human normal and osteoarthritic chondrocyte proteomes. J Proteome Res. 2010 Jun 4;9(6):3035-45. doi: 10.1021/pr901209s. PubMed PMID: 20387907.
  • Ruiz-Romero C, Calamia V, Mateos J, Carreira V, Martínez-Gomariz M, Fernández M, Blanco FJ. Mitochondrial dysregulation of osteoarthritic human articular chondrocytes analyzed by proteomics: a decrease in mitochondrial superoxide dismutase points to a redox imbalance. Mol Cell Proteomics. 2009 Jan;8(1):172-89. doi: 10.1074/mcp.M800292-MCP200. Epub 2008 Sep 9. PubMed PMID: 18784066.


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