Prof. Thierry Arnould  / Prof. Patricia Renard

Unité de Recherche en Biologie Cellulaire
University of Namur,
Namur Research Institute for Life Sciences (NARILIS)

61, rue de Bruxelles, 5000 Namur
Phone:
+32 (0)81724125

Web




Research Lines

  • Analysis of the mitochondrial biogenesis (characterization and role) during the differentiation of different mesenchymal stem cells.
  • Adipocyte cell responses triggered by mitochondrial uncoupling and dysfunction
  • Impact and effects of ER stress or lysosomal storage disorder on mitochondrial morphology and activity.
  • Relation « host-pathogens » between a eukaryotic cell and a facultative intracellular bacteria


Facilities

  • Cell culture facilities and the regular equipment for cell biology and biochemsitry,
  • Real-time PCR for microfluidics cards
  • Confocal microscope and a BD Pathway 855 Imaging Systems
  • A complete proteomic platform for 2D-DIGE analysis and identification of proteins by mass spectrometry (MALDI-TOF and Q-TOF2-LC MS-MS). 
  • The laboratory also belongs to NARILIS (an Institute for life Sciences) and has access to many facilities


Publications

  • Mild mitochondrial uncoupling does not affect mitochondrial biogenesis but downregulates pyruvate carboxylase in adipocytes: role for triglyceride content reduction.
  • Mitochondrial (dys)function in adipocyte (de)differentiation and systemic metabolic alterations.
  • Mild mitochondrial uncoupling induces 3T3-L1 adipocyte de-differentiation by a PPARgamma-independent mechanism, whereas TNFalpha-induced de-differentiation is PPARgamma dependent.
  • CREB activation induced by mitochondrial dysfunction triggers triglyceride accumulation in 3T3-L1 preadipocytes.
  •  Mitochondrial biogenesis in mtDNA-depleted cells involves a Ca2+-dependent pathway and a reduced mitochondrial protein import.
  • Mitochondrial dysfunction induces triglyceride accumulation in 3T3-L1 cells: role of fatty acid beta-oxidation and glucose.
  • CREB activation induced by mitochondrial dysfunction is a new signaling pathway that impairs cell proliferation.


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